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tar-nm-c24  (BioAutomation corporation)

 
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    BioAutomation corporation tar-nm-c24
    a Overlay of the dynamic ensembles of the <t>TAR</t> bulge. b <t>RNA</t> backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).
    Tar Nm C24, supplied by BioAutomation corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tar-nm-c24/tar+nm+c24/pmc07608651-253-3-14
    Average 90 stars, based on 1 article reviews
    tar-nm-c24 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction"

    Article Title: Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction

    Journal: Nature Communications

    doi: 10.1038/s41467-020-19371-y

    a Overlay of the dynamic ensembles of the TAR bulge. b RNA backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).
    Figure Legend Snippet: a Overlay of the dynamic ensembles of the TAR bulge. b RNA backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).

    Techniques Used:

    a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.
    Figure Legend Snippet: a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.

    Techniques Used:

    Related Articles

    other:

    Article Title: Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction
    Article Snippet: TAR, TAR-Nm-U23, and TAR-Nm-C24 RNA samples were synthesized using a MerMade 6 Oligo Synthesizer (BioAutomation) via solid-phase synthesis using standard phosphoramidite chemistry and deprotection protocols.



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    tar-nm-c24  (BioAutomation corporation)
    90
    BioAutomation corporation tar-nm-c24
    a Overlay of the dynamic ensembles of the <t>TAR</t> bulge. b <t>RNA</t> backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).
    Tar Nm C24, supplied by BioAutomation corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tar-nm-c24/tar+nm+c24/pmc07608651-253-3-14
    Average 90 stars, based on 1 article reviews
    tar-nm-c24 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Overlay of the dynamic ensembles of the TAR bulge. b RNA backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).

    Journal: Nature Communications

    Article Title: Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction

    doi: 10.1038/s41467-020-19371-y

    Figure Lengend Snippet: a Overlay of the dynamic ensembles of the TAR bulge. b RNA backbone torsion angles exhibiting different and similar distributions between Anton-MD-NMR and FARFAR-NMR are colored red and green, respectively (“Methods”). c 2D density maps of δ versus γ comparing Anton-MD-NMR and FARFAR-NMR ensembles ( N = 2000) for bulge residues as well as A22 and U40. The bin width is 20°. d Structure of the ribose moiety in C3′- endo and C2′- endo conformations. e Population of C2′- endo pucker at bulge residues as well as A22 and U40 in the FARFAR-library ( N = 10,000, red open), FARFAR-NMR ( N = 20 × 100 = 2000, red fill), Anton-MD library ( N = 10,000, blue open) and Anton-MD-NMR ( N = 20 × 100 = 2000, blue fill). Experimental estimates of the C2′- endo population based on 13 C chemical shifts are indicated above the bars (“Methods”). f The population of conformers in the ensemble as a function of the number of C2′- endo bulge residues for FARFAR-NMR (red, N = 20 × 100 = 2000) and Anton-MD-NMR (blue, N = 20 × 100 = 2000).

    Article Snippet: TAR, TAR-Nm-U23, and TAR-Nm-C24 RNA samples were synthesized using a MerMade 6 Oligo Synthesizer (BioAutomation) via solid-phase synthesis using standard phosphoramidite chemistry and deprotection protocols.

    Techniques:

    a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.

    Journal: Nature Communications

    Article Title: Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction

    doi: 10.1038/s41467-020-19371-y

    Figure Lengend Snippet: a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.

    Article Snippet: TAR, TAR-Nm-U23, and TAR-Nm-C24 RNA samples were synthesized using a MerMade 6 Oligo Synthesizer (BioAutomation) via solid-phase synthesis using standard phosphoramidite chemistry and deprotection protocols.

    Techniques: